'real-time PCR': which machine to buy??

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Tue Feb 8 06:03:08 EST 2000


In article <rh-0802001031340001 at a6-curtis.ibls.gla.ac.uk>, Robert
Hartley <rh at mblab.gla.ac.uk> writes
>I use a RapidCycler from Idaho
>technologies and if the Roche machine uses the same mounting then you can
>set up a reaction in a 96 well microtitre plate (elisa plate) and draw up
>8 to 16 at a time and seal with the flame thrower.

Different in that each capillary is sealed at one end with a sort of
lens at the sealed end (acts as a light pipe) and has a plastic bit at
the other. You pipette in the reaction mix, partially push in a plastic
plug, microfuge briefly and push the plug fully in to seal. These then
get placed in what looks like a microfuge rotor on the machine itself. 

Of course push to hard when capping and you break the capillary (or one
used to on the original more fragile Idaho capillaries - not the thicker
wall Roche ones). Alternatively one would briefly microfuge and the
capillary would part company with the plastic bit at the top. 

So if you use nasties and are worried about sharps, don't use a
LightCycler! 

Has anyone yet managed to demo a Biorad i-Cycler or the Corbett
Rotagene? If so do they work?

Duncan   
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk




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