can I make my own poly dT primer

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Tue Feb 8 07:41:19 EST 2000


In article <20000208114040.27941.qmail at web3407.mail.yahoo.com>, sam
wayne <yilao at yahoo.com.cn> writes
>May I ask a question here? It might appear too simple
>but I am having problem of solving it.
>When I do RT-PCR, I use SuperscriptII and aslo their
>poly dT primer.
>Can I make my own poly dT primer,

Not a problem, just place an order with your local DNA synthesis
company.

Order straight dT(18) or an anchor primer dT(18)VN where V is any base
but T and N is, well N. Whilst you are at it you could order some random
hexamers dN(6) or nonamers dN(9), as these may also be helpful

>provided the randomness?

?

>Do I need to phosphorylate
>it?

No.

Duncan

-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk




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