Glycerol gradients for protein purification

Sean Patterson seanpat at
Tue Feb 8 15:35:47 EST 2000

Posted for a friend:

Does anybody have a detailed protocol to perform and run glycerol gradients
(12-step, 25-50%) to isolate protein complexes?  How should I solubilize the
samples? (supposed to be membrane proteins, but not sure).  Thanks!


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