another question on DNA-quatitizing

Jered J Floyd jered at mit.edu
Wed Feb 9 00:42:51 EST 2000


aloiv at my-deja.com writes:

> Then, if the manufacturer adjusts the concentrations for each sized
> bp fragment equal, will all of the ladder bands fluoresce in equal
> intensity (after EtBr staning and UV illumination) -- for example,
> 100bp band and 3000bp band with equal brightness?

They ought to, since the calibration is in ng of DNA, not in fmol.
The calibration on the ladders is fragment size independent.

--Jered




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