Trivial transformation improvement

[Frederik Börnke]

Louis Hom schrieb:
> 
> Maybe folks do this already and I just didn't know, but my labmate and I
> have seen a nice bump in transformation efficiency when cells are allowed
> to recover in a 37 deg incubator or on top of the floating rack in the 37
> deg water bath instead of being set into the water like normal.
> --
> ______________________________________________________________________________
> Lou Hom >K'93
> lhom at ocf.berkeley.edu
> http://www.ocf.berkeley.edu/~lhom/

Hi Lou,

You mean the recovery step subsequent to the heat shock? When I look
up my protocols it is always done at 37 deg.

Cheers
Ricky
 
*******************************************************************

Frederik Boernke
Research Group of  Molecular Plant Physiology
Institute for Plant Genetics and Crop Plant Research (IPK)
Corrensstr. 3
06466 Gatersleben
Tel.  039482 -5 321
Fax. 039482 -5 515
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