Trivial transformation improvement

Louis Hom lhom at OCF.Berkeley.EDU
Wed Feb 9 02:37:28 EST 2000


In article <38A10CCE.96ABFCA at gmx.net>,
Frederik =?iso-8859-1?Q?B=F6rnke?=  <ricky_boernke at gmx.net> wrote:
>
>You mean the recovery step subsequent to the heat shock? When I look
>up my protocols it is always done at 37 deg.

	Right, after you add the media and before plating.  It seems like
a slower warming (in 37 deg air -- either in an incubator or in the air
above the water in a 37 deg water bath) gives us better results than
sticking the tubes directly into the 37 deg water.
-- 
______________________________________________________________________________
Lou Hom >K'93			     
lhom at ocf.berkeley.edu		
http://www.ocf.berkeley.edu/~lhom/ 	    




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