quantitizing DNA from gel-electrophoresis

Richard P. Grant rgrant at netscape.net
Wed Feb 9 03:09:28 EST 2000


In article <38A055E4.4EEC723F at cc.umanitoba.ca>, Rob Kirkpatrick 
<kirkpat at cc.umanitoba.ca> wrote:

<...>
> run a gel where you load a range of known
> quantities of DNA (say digested lambda DNA or something that has been
> accurately spec'd) then keep the picture handy when you try to guesstimate
> unknown samples.

Far safer to run a known on every gel with unknowns - compensates for 
differences in effective [EtBr], gel thickness, day of the week &c.

R

-- 
Richard P. Grant MA DPhil          
Structural Studies Group, MRC-LMB
http://www2.mrc-lmb.cam.ac.uk/personal/rpg/index.html
Please reply to rpg 'at' mrc-lmb.cam.ac.uk




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