another question on DNA-quatitizing

Richard P. Grant rgrant at netscape.net
Wed Feb 9 03:11:30 EST 2000


In article <87qovg$lef$1 at nnrp1.deja.com>, aloiv at my-deja.com wrote:

> will all of the ladder
> bands fluoresce in equal intensity (after EtBr staning and UV
> illumination) -- for example, 100bp band and 3000bp band with equal
> brightness?

To be absolutely clear, here;

If you have the same number of molecules of a 100 bp fragment as a 3000 
bp fragment, then you will get more fluorescence from the larger band.

R

-- 
Richard P. Grant MA DPhil          
Structural Studies Group, MRC-LMB
http://www2.mrc-lmb.cam.ac.uk/personal/rpg/index.html
Please reply to rpg 'at' mrc-lmb.cam.ac.uk




More information about the Methods mailing list