BL21(DE3) and trc promoter

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Wed Feb 9 04:09:15 EST 2000


In article <Pine.GSO.4.21.0002090952300.8351-100000 at sun3.oulu.fi>, Petri
Kursula <pkursula at cc.oulu.fi> writes
>Is this a cell line suitable for expression in general, or does it
>have a bunch of nasty proteases?

I would work on the assumption that all E.coli's have a bunch of
proteases. Whether they are nasty will depend upon your protein. I'm
sure that maybe in the last 3-5 years there was a paper in Nature
Biotechnology where the E.coli proteases were gradually all knocked out.
If you can find it that may help you make a more informed decision as to
which strain to gamble on.

Duncan 
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk




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