BL21(DE3) and trc promoter

Dr. Duncan Clark Duncan at
Wed Feb 9 04:09:15 EST 2000

In article <Pine.GSO.4.21.0002090952300.8351-100000 at>, Petri
Kursula <pkursula at> writes
>Is this a cell line suitable for expression in general, or does it
>have a bunch of nasty proteases?

I would work on the assumption that all E.coli's have a bunch of
proteases. Whether they are nasty will depend upon your protein. I'm
sure that maybe in the last 3-5 years there was a paper in Nature
Biotechnology where the E.coli proteases were gradually all knocked out.
If you can find it that may help you make a more informed decision as to
which strain to gamble on.

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

More information about the Methods mailing list