ho to make a probe??

heehalloo at my-deja.com heehalloo at my-deja.com
Wed Feb 9 09:46:06 EST 2000


Hello,

I'm looking for a method to prepare a probe
(rather to purify the probe).
In a reaction with T4-kinase I label my DNA.
Then I want to purify it over a little column
(Sephadex G50). How?
Bring it on the column (1 ml), wash with 200
microliter MQ) and elute with 300 microliter??
Is this realistic?

Robbie


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