ho to make a probe??

Hiranya S. Roychowdhury hroychow at nmsu.edu
Wed Feb 9 10:41:15 EST 2000


Did you look for the protocol in any standard mol bio lab manual?

At 02:46 PM 2/9/00 GMT, heehalloo at my-deja.com wrote:
>Hello,
>
>I'm looking for a method to prepare a probe
>(rather to purify the probe).
>In a reaction with T4-kinase I label my DNA.
>Then I want to purify it over a little column
>(Sephadex G50). How?
>Bring it on the column (1 ml), wash with 200
>microliter MQ) and elute with 300 microliter??
>Is this realistic?
>
>Robbie
>
>
>Sent via Deja.com http://www.deja.com/
>Before you buy.
>


Dr. Hiranya Sankar Roychowdhury
GENE LAB/ EPPWS
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu

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