Trivial transformation improvement

Rick Bright rbright at emory.edu
Wed Feb 9 13:25:56 EST 2000


My transformation heatshocks are done at 42 deg C.  From ice, then back
to ice.  Where is the 37 deg C coming from for heatshock?

Frederik Börnke wrote:
> 
> Louis Hom schrieb:
> >
> > Maybe folks do this already and I just didn't know, but my labmate and I
> > have seen a nice bump in transformation efficiency when cells are allowed
> > to recover in a 37 deg incubator or on top of the floating rack in the 37
> > deg water bath instead of being set into the water like normal.
> > --
> > ______________________________________________________________________________
> > Lou Hom >K'93
> > lhom at ocf.berkeley.edu
> > http://www.ocf.berkeley.edu/~lhom/
> 
> Hi Lou,
> 
> You mean the recovery step subsequent to the heat shock? When I look
> up my protocols it is always done at 37 deg.
> 
> Cheers
> Ricky
> 
> *******************************************************************
> 
> Frederik Boernke
> Research Group of  Molecular Plant Physiology
> Institute for Plant Genetics and Crop Plant Research (IPK)
> Corrensstr. 3
> 06466 Gatersleben
> Tel.  039482 -5 321
> Fax. 039482 -5 515
> http://www.ipk-gatersleben.de




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