another question on DNA-quatitizing
rashie at bio.umass.edu
Wed Feb 9 18:51:10 EST 2000
something else to keep in mind with respect to the point about fluorescing
with equal intensity is fact that the 100 bp band, since it has travelled a
longer distance in the gel, would be more diffuse than the 3000 bp
band. one would then have to think in terms of a dilution series to build
a saturation curve from which you can quantify your unknowns.
At 03:11 AM 2/9/00 , you wrote:
>In article <87qovg$lef$1 at nnrp1.deja.com>, aloiv at my-deja.com wrote:
> > will all of the ladder
> > bands fluoresce in equal intensity (after EtBr staning and UV
> > illumination) -- for example, 100bp band and 3000bp band with equal
> > brightness?
>To be absolutely clear, here;
>If you have the same number of molecules of a 100 bp fragment as a 3000
>bp fragment, then you will get more fluorescence from the larger band.
>Richard P. Grant MA DPhil
>Structural Studies Group, MRC-LMB
>Please reply to rpg 'at' mrc-lmb.cam.ac.uk
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