another question on DNA-quatitizing

rashie athukorala rashie at bio.umass.edu
Wed Feb 9 18:51:10 EST 2000


something else to keep in mind with respect to the point about fluorescing 
with equal intensity is fact that the 100 bp band, since it has travelled a 
longer distance in the gel, would be more diffuse than the 3000 bp 
band.  one would then have to think in terms of a dilution series to build 
a saturation curve from which you can quantify your unknowns.



At 03:11 AM 2/9/00 , you wrote:
>In article <87qovg$lef$1 at nnrp1.deja.com>, aloiv at my-deja.com wrote:
>
> > will all of the ladder
> > bands fluoresce in equal intensity (after EtBr staning and UV
> > illumination) -- for example, 100bp band and 3000bp band with equal
> > brightness?
>
>To be absolutely clear, here;
>
>If you have the same number of molecules of a 100 bp fragment as a 3000
>bp fragment, then you will get more fluorescence from the larger band.
>
>R
>
>--
>Richard P. Grant MA DPhil
>Structural Studies Group, MRC-LMB
>http://www2.mrc-lmb.cam.ac.uk/personal/rpg/index.html
>Please reply to rpg 'at' mrc-lmb.cam.ac.uk


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