Trivial transformation improvement

Frederik Börnke ricky_boernke at gmx.net
Thu Feb 10 01:49:50 EST 2000



Rick Bright schrieb:
> 
> My transformation heatshocks are done at 42 deg C.  From ice, then back
> to ice.  Where is the 37 deg C coming from for heatshock?
> 

[snip]

Yeah, but after back to the ice you probably do a recovery step at 37
deg, 
don't you?
BTW I do my recovery step usually in a shaker not in a waterbath.

Cheers
Ricky




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