Trivial transformation improvement

Nicolas Lemee Lemee at attglobal.net
Thu Feb 10 10:50:31 EST 2000


At 100ug/ml Amp and 30ug/ml Kan final, 30' shake @ 37C works every time.
--
Nicolas

Rafael Maldonado wrote:

> "Dr. Duncan Clark" wrote:
> > I've also seen it said that with Amp one needs recovery but for
> > Kan one doesn't.
>
> Well, it could be the reverse, but I can tell you, Kan need one hour
> recovery, the antibiotic is so strong that without the proper recovery
> the cells will not survive.
>
> Anyway, it sounds a kind of magic to me...
>
> -Rafa
>
> --
> Rafael Maldonado
> Divison of Genetics
> University of Alicante (Spain)





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