Trivial transformation improvement
rbright at emory.edu
Thu Feb 10 11:19:51 EST 2000
Frederik Börnke wrote:
> Rick Bright schrieb:
> > My transformation heatshocks are done at 42 deg C. From ice, then back
> > to ice. Where is the 37 deg C coming from for heatshock?
> Yeah, but after back to the ice you probably do a recovery step at 37
> don't you?
> BTW I do my recovery step usually in a shaker not in a waterbath.
Now I am with you, yes, I do the recovery in a shaker. I don't go near a
waterbath for my transformations except for the 45 sec at 42 deg C. So,
I guess this tip is not valid for the shakers?
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