Trivial transformation improvement

Rick Bright rbright at emory.edu
Thu Feb 10 11:19:51 EST 2000



Frederik Börnke wrote:
> 
> Rick Bright schrieb:
> >
> > My transformation heatshocks are done at 42 deg C.  From ice, then back
> > to ice.  Where is the 37 deg C coming from for heatshock?
> >
> 
> [snip]
> 
> Yeah, but after back to the ice you probably do a recovery step at 37
> deg,
> don't you?
> BTW I do my recovery step usually in a shaker not in a waterbath.
> 
> Cheers
> Ricky


Now I am with you, yes, I do the recovery in a shaker. I don't go near a
waterbath for my transformations except for the 45 sec at 42 deg C.  So,
I guess this tip is not valid for the shakers?




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