Trivial transformation improvement

[Nicolas Lemee]

Of course I meant final concentrations on plates.

I always thought of the pre-plating incubation as a necessary step to allow
the bugs with plasmids to produce whatever makes them grow on a selective
media.
--
Nicolas


Rick Bright wrote:

> Confused me again.  I do not add any antibiotic to the small (80ul) of
> LB or SOB that I put on the bacteria after heatshock while shaking for
> an hour.  The antibiotic is only on the plates.  Am I missing something
> again?
>
> Rick