Trivial transformation improvement

Nicolas Lemee Lemee at
Thu Feb 10 13:16:20 EST 2000

Of course I meant final concentrations on plates.

I always thought of the pre-plating incubation as a necessary step to allow
the bugs with plasmids to produce whatever makes them grow on a selective

Rick Bright wrote:

> Confused me again.  I do not add any antibiotic to the small (80ul) of
> LB or SOB that I put on the bacteria after heatshock while shaking for
> an hour.  The antibiotic is only on the plates.  Am I missing something
> again?
> Rick

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