Best bacteria for mammalian protein expression

Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de
Thu Feb 10 14:39:54 EST 2000


Gareth <gphillip at peds.bsd.uchicago.edu> wrote:
> Anyone heard of a bacterial strain that's been enhanced for mammalian
> protein expression with tRNA's more suitable for the human codon
> frequency.

Yes, there are modified BL21 sold by Stratagene. I don't know whether
they influence the protein expression considerably; this might depend
on your protein of choice.

--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at rzbox.uni-wuerzburg.de  SP4 */
/* "Science is the game we play with God to find out what His rules are."  */




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