Trivial transformation improvement
klenchin at facstaff.REMOVE_TO_REPLY.wisc.edu
Thu Feb 10 20:21:11 EST 2000
:In article <38A25F8E.E25F66D1 at gmx.net>, Frederik BЖrnke
:<ricky_boernke at gmx.net> writes
:>Yeah, but after back to the ice you probably do a recovery step at 37
:>BTW I do my recovery step usually in a shaker not in a waterbath.
:The same applies to electroporation. What is the best temperature,
:treatment and time for post-recovery? I've seen all sorts in the
:literature from RT to 37C to shaking/non-shaking from 10 mins to 60
:mins. I've also seen it said that with Amp one needs recovery but for
:Kan one doesn't.
How is that possible? In either case, don't you need some "recovery"
time to actually make enough protein that confers resistance?
More information about the Methods