In vivo Th determination by DTH assay

Antonieta Herrera amherrera at cigb.edu.cu
Thu Feb 10 21:52:08 EST 2000


Hello netters!!!

I was wondering if some one have news about some kind of DTH 
assay for CD4+ response detection in vivo, by administering an 
MHC II-restricted peptide, just like the assay described by 
Zynkernagel and co-workers in 1992 for CTL response 
determination in vivo (but in that case, the method was reported 
employing a cytotoxic peptide for re-stimulation).
I will also thank any comment about the idea for detecting in vivo T 
helper immune responses using DTH, by stimulating with a helper 
peptide.

Thanks in advance

Ernest
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