Genomic DNA (the Maniatis way) for telomere analysis?

Hastings, Dr R.A. rah22 at
Fri Feb 11 06:54:15 EST 2000

A colleague of mine is hoping to extract DNA from tissue, digest the DNA
with a four-cutter and run the DNA on PFGE to look at telomere size. I've
got a method from Maniatis which allows preparation and digestion in an
agarose plug prior to running the gel. Have any of you successfully used
this or a similar method for telomere analysis? Also what's the best way to
quantitate the DNA prior to loading on the gel?

Thanks in advance for any help you can give me,


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