detatch the protein from the resin

Yong Jiang yjiang at ucsd.edu
Mon Feb 14 18:17:46 EST 2000


Hi, 
I express the GST fustion protein by pGEX vector and cleaved the fusion protein in the resin, but can not get the target protein, even the GST. When I ran the resin on the SDS gel, I found that both GST and my protein stayed in the resin. Does anyone have some suggestion to get the target protein off?
Any suggestion is appreciated.
Thank you very much.
Yong 


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