detatch the protein from the resin

Yong Jiang yjiang at
Mon Feb 14 18:17:46 EST 2000

I express the GST fustion protein by pGEX vector and cleaved the fusion protein in the resin, but can not get the target protein, even the GST. When I ran the resin on the SDS gel, I found that both GST and my protein stayed in the resin. Does anyone have some suggestion to get the target protein off?
Any suggestion is appreciated.
Thank you very much.

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