where do you purchase your restriction enzymes?

[Cornelius Krasel]

Frederik Börnke <ricky_boernke at gmx.net> wrote:
> We regularly buy our 
> restriction enzymes from Boehringer 'cause we made some
> bad experiences with enzymes from other suppliers. Since our
> group is expanding rapidly and cloning work increases we are
> in search of alternatives especially for the rather expensive
> REs like NotI, NcoI etc.. I found NotI to be 8 times less 
> expensive at NEB's so I'd like to give it a try. Where do you
> get your enzymes from?

NEB. They work, even in the Boehringer (err, Roche Diagnostics :-)
buffers (the Boehringer buffer system is IMO more convenient than
the NEB one).

--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at rzbox.uni-wuerzburg.de  SP4 */
/* "Science is the game we play with God to find out what His rules are."  */