where do you purchase your restriction enzymes?

Frederik Börnke ricky_boernke at gmx.net
Tue Feb 15 09:09:57 EST 2000



Jered J Floyd schrieb:
> 
> Most of our REs are from NEB. They're particularly convenient given we
> are in Cambridge; we can sometimes get orders the same day. We are
> satisfied with their products, and they have a good selection, (It's like
> Pokemon...gotta collect them all!)
> 
> Personally, I just had a bad experience with NotI. Even at a 20-fold
> overdigest I found it was truly pitiful at cutting supercoiled DNA. (I
> wouldn't blame NEB for this, of course. They do mention it's bad at
> cutting supercoiled, but they could stress it a bit more.)
> 
> --Jered

We just had the same problem with NotI from Roche. How did you get
around?

Ricky




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