where do you purchase your restriction enzymes?

[Hiranya S. Roychowdhury]

Our experience has been that RE's work with buffers from different suppliers
equally well.
NEB is a very good and cheaper source of many common and uncommon RE's. We
also use BRL's without problems.
I have personally substituted various buffers wih Pharmacia's "One-Phor-All
plus" (with their recommendations) and have seldom encountered problems.

At 10:27 AM 2/15/00 +0100, Frederik Börnke wrote:
>Dear all,
>
>I have some kind of general question. We regularly buy our 
>restriction enzymes from Boehringer 'cause we made some
>bad experiences with enzymes from other suppliers. Since our
>group is expanding rapidly and cloning work increases we are
>in search of alternatives especially for the rather expensive
>REs like NotI, NcoI etc.. I found NotI to be 8 times less 
>expensive at NEB's so I'd like to give it a try. Where do you
>get your enzymes from? And how are they in respect to compatibility
>with enzymes from other suppliers? For instance, we used to have
>some enzymes from Amersham but made the experience that most
>of them are not compatible with the ones from Boehringer with
>respect to the buffer. Any thoughts?
>
>TIA
>Ricky
> 
>*******************************************************************
>
>Frederik Boernke
>Research Group of  Molecular Plant Physiology
>Institute for Plant Genetics and Crop Plant Research (IPK)
>Corrensstr. 3
>06466 Gatersleben
>Tel.  039482 -5 321
>Fax. 039482 -5 515
>http://www.ipk-gatersleben.de
>


Dr. Hiranya Sankar Roychowdhury
GENE LAB/ EPPWS
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu

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