RNA from <100 mg leaf tissue

Karthik Aghoram kaghoram at garnet.acns.fsu.edu
Tue Feb 15 13:29:34 EST 2000


A really neat protocol that has always worked for me (in Vicia) is from
Wadsworth et al, 1989 (I think the year is right).  I'll check on that
reference and get back.

 


Ivo Rieu (irieu at sci.kun.nl) wrote:
: Hello,

: I want to do RNA extraction from individual Arabidopsis thaliana plants,
: less than 100 mg total fresh weight.
: If possible DNA isolation at the same time.
: Does anyone have a protocol/kit/idea??

: Thanks





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