Luciferase variation among replicates

T Cheng pcheng18 at hotmail.com
Tue Feb 15 23:58:13 EST 2000


Hi netter, 

I am using the luciferase assay kit to measure the luciferase activity of transfected primary culture of oyster cells in 96 well plate. The luminometer were used. 

However, the luciferase activity were vary among 6 replicates of the same treatment. The variation of luciferase activity were vary 100 folds from 0.002 light unit (background) to 0.2 among 6 replicates. 

What are the possible cuases for this variation? 

Thanks 

Philip

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