PCR from blood dots on paper ?
hiltunen at csc.fi
Wed Feb 16 01:57:01 EST 2000
We have blood dots (two 1-cm dots from each subject) on
paper and aim to do PCR with them. I ask for your help in
deciding the best way to proceed, since even the Red Book
does not tell the right procedure. Two options come into my mind:
1) We (this means the technician ;) excise a small (1mm x 1mm)
piece of paper for each PCR reaction and start the reaction
with prolonged denaturation. Is the 9-min 95°C used with
AmpliTaq Gold sufficient ?
2) We incubate the whole blood dot in 1xPCR buffer and use an
aliquot in the PCRs. Does this require boiling or something
else? How is the DNA stored? Any tricks in PCR parameters?
Any help is appreciated.
With best regards,
Dept of Medicine
University of Helsinki
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