undigestable genomic DNA

Gregor Bucher st2153 at zi.biologie.uni-muenchen.de
Wed Feb 16 09:30:02 EST 2000

Hi southern-freaks!

I want to do a southern with genomic DNA (insect). When I digest the DNA
with Hind3 it looks fine. With EcoR1 and Xho however, there is quiete a
bit high molecular DNA that obviously has not been digested (digesting 2
hours or o/n does not change things). Does anybody have experience with
genomic southerns?

Should I proceed or start with a new preparation of DNA? 

Thanks for comments!

Gregor Bucher, Zoologisches Institut der LMU München
st2153 at zi.biologie.uni-muenchen.de

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