Promoter activity

Dr. Fernando Rodriguez-Pascual frodrigu at mail.uni-mainz.de
Wed Feb 16 10:28:14 EST 2000


Hallo,

What kind of plasmid is your "insertless" control? Does it have already
any promoter sequence that masks the effect of your insert? You can try
plasmids with lower basal activity (I use, for example, pGL3basic
(Promega) and the basal activity is very low). On the other hand, as you
find really high activity, it may be possible that your system is
saturated, you may try transfection with less DNA. In the worse case, it
can be that your putative regulatory sequence does not show promoter
activity...

Bye

Fernando
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Fernando Rodríguez Pascual
Department of Pharmacology
Johannes Gutenberg University
Obere Zahlbacher Str. 67
D-55101 Mainz, Germany
Tf. +49 (6131) 39 3 68 36 or 39 3 32 45
Fax. +49 (6131) 39 3 66 11
email: frodrigu at mail.uni-mainz.de
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