cloning the wrong way?

Richard P. Grant rgrant at netscape.net
Wed Feb 16 11:55:39 EST 2000


In article <38AAD3B4.D212C32C at uni-konstanz.de>, 
Frank.Fackelmayer at uni-konstanz.de wrote:

> ALWAYS!!! Especially when using Taq polymerase which is notorious for
> making mistakes.

Pish.

To the second part of this, anyway.  Drop the dNTP conc to 50 uM (from 
200 uM) and watch your error rate fall away.

R

-- 
Richard P. Grant MA DPhil          
Structural Studies Group, MRC-LMB
http://www2.mrc-lmb.cam.ac.uk/personal/rpg/index.html
Please reply to rpg 'at' mrc-lmb.cam.ac.uk




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