cloning the wrong way?

Richard P. Grant rgrant at
Wed Feb 16 11:55:39 EST 2000

In article <38AAD3B4.D212C32C at>, 
Frank.Fackelmayer at wrote:

> ALWAYS!!! Especially when using Taq polymerase which is notorious for
> making mistakes.


To the second part of this, anyway.  Drop the dNTP conc to 50 uM (from 
200 uM) and watch your error rate fall away.


Richard P. Grant MA DPhil          
Structural Studies Group, MRC-LMB
Please reply to rpg 'at'

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