cloning the wrong way?
Richard P. Grant
rgrant at netscape.net
Wed Feb 16 11:55:39 EST 2000
In article <38AAD3B4.D212C32C at uni-konstanz.de>,
Frank.Fackelmayer at uni-konstanz.de wrote:
> ALWAYS!!! Especially when using Taq polymerase which is notorious for
> making mistakes.
To the second part of this, anyway. Drop the dNTP conc to 50 uM (from
200 uM) and watch your error rate fall away.
Richard P. Grant MA DPhil
Structural Studies Group, MRC-LMB
Please reply to rpg 'at' mrc-lmb.cam.ac.uk
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