dissolving biotinylated oligo
dolly at sheep.shaggers.on.the.road
Wed Feb 16 16:20:08 EST 2000
Frank O. Fackelmayer <Frank.Fackelmayer at uni-konstanz.de> wrote in message
news:38A1778D.3376B0E7 at uni-konstanz.de...
> Hi all,
> I got a quite trivial problem and hope someone has an idea. I have
> ordered a synthetic oligonucleotide, a GC-rich 12mer with 3´ biotin.
> What I received was 14.3 OD of lyophilized (yellowish) material that
> does not dissolve well. I first tried dissolving in water at 250pmol/ul,
> which did not work. I then doubled the volume, but there was still quite
> a lot of undissolved material even after heating to 70 degrees. So I
> added DMSO to 20% final concentration, but still.... I ordered the oligo
> in a quite high quality, HPSF, and I haven´t had any problems with the
> primers made by this company. So I think the problem is the 3´ biotin,
> or the GC-richness. The sequence is 5´GGGCGGCGACCT-biotin.
> Any suggestions?
Biotin is not the problem. The guanines are the problem. Some more DMSO and
heating to 95 degrees might solve your problem. Addition of salt might also
enhance the solubility of your oligo.
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