Alternatives to Dextran Sulphate

Hiranya S. Roychowdhury hroychow at
Wed Feb 16 18:56:23 EST 2000

No need to use that stuff in Southerns (or northerns, for that matter). If
you are not sure about leaving dextran sulphate out of the usual "Denhardt's
rgt containing" buffer, switch to Church & Gilbert's plain phosphate-SDS
buffer. It works very nicely (better, if you ask me) for both Southern and
northern hybridizations.

At 11:22 PM 2/16/00 -0000, Livinus.Emebiri at wrote:
>Does anyone know if there are any alternatives to the use of Dextran
Sulphate in
>hybridisation buffer of southern blots?

Dr. Hiranya Sankar Roychowdhury
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at


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