cloning the wrong way?

Richard P. Grant rgrant at netscape.net
Thu Feb 17 06:37:44 EST 2000


In article <38ABBEE2.6FB637C4 at solea.quim.ucm.es>, Sergio 
<sergioal at solea.quim.ucm.es> wrote:

> Sounds interesting, since i have lots of PCR clonings to do (and i always 
> get a
> mutation rate around 2/1000). Do i need to change any parameter in the 
> PCR
> conditions if i use 50 uM dNTP?


You may have to re-titrate the Mg++ (for obvious reasons).
Good luck with it, but as ever anything I say is based on my own 
experience and YMMV.

R

-- 
Richard P. Grant MA DPhil          
Structural Studies Group, MRC-LMB
http://www2.mrc-lmb.cam.ac.uk/personal/rpg/index.html
Please reply to rpg 'at' mrc-lmb.cam.ac.uk




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