Amplitaq Gold in RapidCycler!

Csaba Kiss csakis at ki.se
Thu Feb 17 06:49:36 EST 2000


Hi!
We are using a Rapid cycler from Idahotec. I used Platinum Taq polymerase to
do hot start PCR before and it works perfectly. Now we have a very good
price offer from PE to use Amplitaq Gold. If you want to use the Gold you
have to keep your sample at 95 degree for 10 minutes to activate the enzyme.
In the Rapid Cycler, however, the denaturation step is very short, ususally
0 sec. Is it enough to activate the enzyme fully, even if I insert a 10
minute 95 degree initial incubation?

Csaba






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