Contamination with Nucleic Acids

rogier stugerNOstSPAM at cellbiology.uni-frankfurt.de.invalid
Thu Feb 17 07:53:28 EST 2000


Hi Carmelo,
incubate with DNase I or RNase A for half an hour or so. if this
doesn't help, your protein is binding DNA/RNA so tight that the
nucleases can't bite 'em anymore. then you'll have to release the
D/RNA from the protein by heat, salt, SDS, whatever. of course,
the disadvantage is that this will denature your protein...
Good luck,
Rogier


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