Promoter activity

Theodosios Kyriakou theodosios73 at my-deja.com
Thu Feb 17 13:44:10 EST 2000


In article <38AAC20D.38DC at mail.uni-mainz.de>,
  frodrigu at mail.uni-mainz.de wrote:
> Hallo,
>
> What kind of plasmid is your "insertless" control?

My insertless control is pCAT3-basic (promega).

Does it have already
> any promoter sequence that masks the effect of your insert?


I would guess not.This plasmid is designed especially for reporter gene
assays but i guess you can never exclude anything....

You can try
> plasmids with lower basal activity (I use, for example, pGL3basic
> (Promega) and the basal activity is very low). On the other hand, as
you
> find really high activity, it may be possible that your system is
> saturated, you may try transfection with less DNA.

I agree that this is a possibility


In the worse case, it
> can be that your putative regulatory sequence does not show promoter
> activity...

The strange thing is that they show barely less activity than the
insertless control....
>
> Bye
>
> Fernando
> --
> #############################################
> Fernando Rodríguez Pascual
> Department of Pharmacology
> Johannes Gutenberg University
> Obere Zahlbacher Str. 67
> D-55101 Mainz, Germany
> Tf. +49 (6131) 39 3 68 36 or 39 3 32 45
> Fax. +49 (6131) 39 3 66 11
> email: frodrigu at mail.uni-mainz.de
> #############################################
>

--

Theodosios Kyriakou
Biological Sciences,WarwickUniversity ,CV4 7AL
tel:++44-1203-522440,++44-1203-572572 ext.30164


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