Promoter activity

Theodosios Kyriakou theodosios73 at my-deja.com
Thu Feb 17 13:53:51 EST 2000


In article <38AB3597.76FB at le.ac.uk>,
  "A.F. Simpson" <AFS7 at le.ac.uk> wrote:
> Theodosios Kyriakou wrote:
>
> Hi, Theo!
Hello there

>
> Which plasmid is it?

pCAT-basic (promega)
> Firstly, have you tried a CAT assay on mock transfected 293s (no
plasmid
> at all, just lipid and the same treatment as the trasnfected cells),
> just to make sure it's not the assay step which is giving the weird
> results?

Yes i did.No problems there

> Secondly, which 293 cells are you using?  As well as the ordinary ones
> there are some of my stocks in the liq N2 which came stably
transfected
> with an EBNA gene and which I suppose might behave oddly although
> off-hand I can't think of a reason why they should.

I am using the ordinary ones but the EBNA ones have no problems as well.

Theodosios Kyriakou
Biological Sciences,WarwickUniversity ,CV4 7AL
tel:++44-1203-522440,++44-1203-572572 ext.30164


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