undigestable genomic DNA

Rafael Maldonado rmaldonado at ua.es
Fri Feb 18 05:35:15 EST 2000

> Gregor Bucher wrote:
> > Hi southern-freaks!
> >
> > I want to do a southern with genomic DNA (insect). When I digest the DNA
> > with Hind3 it looks fine. With EcoR1 and Xho however, there is quiete a
> > bit high molecular DNA that obviously has not been digested (digesting 2
> > hours or o/n does not change things). Does anybody have experience with
> > genomic southerns?

You could add spermidine, sometimes it is an improvement. 

Rafael Maldonado
Divison of Genetics
University of Alicante (Spain)

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