What gel & conditions to use ?

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Fri Feb 18 07:32:59 EST 2000


In article <38AD09A4.815E2B8E at clo.fgov.be>, Jan De Riek
<j.deriek at clo.fgov.be> writes
>    Now we use 7.5 M urea, 6% PAA, 1X TBE on 48 cm SequiGen Sequencing 
>    Cell from BIORAD. 

I would expect you to have to lower the acrylamide concn., say down to
3 or 4% to cover up to 3000bp. The problem will then be that you lose
resolution down at the 100bp end.

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk




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