What gel & conditions to use ?

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Fri Feb 18 07:32:59 EST 2000

In article <38AD09A4.815E2B8E at clo.fgov.be>, Jan De Riek
<j.deriek at clo.fgov.be> writes
>    Now we use 7.5 M urea, 6% PAA, 1X TBE on 48 cm SequiGen Sequencing 
>    Cell from BIORAD. 

I would expect you to have to lower the acrylamide concn., say down to
3 or 4% to cover up to 3000bp. The problem will then be that you lose
resolution down at the 100bp end.

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

More information about the Methods mailing list