Alternatives to Dextran Sulfate in ISH

[George Flentke]

>sulfate in Northern/Southern blots. We recently noted that Dextran
>Sulfate is the major cause for background in our in situs with DIG and
>fluorescein labeled RNA probes.
>We're currently checking whether this may be related to different
>batches or manufacturers.

We only used Pharmacia.  Other brands were worse.  I would be happy with 
any kind of "purification protocol" to decrease the problem.

George