Alternatives to Dextran Sulfate in ISH

George Flentke gflentke at facstaff.wisc.edu
Fri Feb 18 06:36:25 EST 2000


>sulfate in Northern/Southern blots. We recently noted that Dextran
>Sulfate is the major cause for background in our in situs with DIG and
>fluorescein labeled RNA probes.
>We're currently checking whether this may be related to different
>batches or manufacturers.

We only used Pharmacia.  Other brands were worse.  I would be happy with 
any kind of "purification protocol" to decrease the problem.

George




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