EGFP antibody Western

Frank O. Fackelmayer Frank.Fackelmayer at uni-konstanz.de
Fri Feb 18 11:16:07 EST 2000


Martin Offterdinger wrote:

> Hi!
> Are there any experiences with Clontech s a-GFP polyclonal AB in Westerns;
> They say it is suitable for westerns, but a get a very large number of bands
> that make it impossible to distinguish which is the right one. Does one have
> to use special blocking conditions or an extremely high dilution to achieve
> some specificity?
> Is there anyone who has successfully used it for Western (Clontech, Cat #
> 8367-1)
> Thank you in advance

Hi Martin,
Not really an answer to your question, but we´ve been using the GFP antibody
from Roche (former Boehringer Mannheim; no affiliations!) with great success.
It is a mixture of two monoclonals, and is extremely specific: only one band in
western blots with no especially harsh conditions. If you cannot get the other
antibody to work properly, give this one a try.

Frank






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