strange cloning results

Yong Jiang yjiang at ucsd.edu
Fri Feb 18 15:42:12 EST 2000


Hi,
What I think is your frag is too much, may be you need to lower the frag's
content.
Yong
Martin Hoehne <marthoe at REMOVE.biologie.uni-freiburg.de> wrote in message
news:38AD4D7E.50FD723F at biologie.uni-freiburg.de...
> Hello everybody,
>
> I cloned a SalI / XhoI fragment into a vector cut with SalI and treated
> with CIP.
>
> What I expected was to get 3 different kinds of clones:
> - insert in correct orientation
> - insert in false orientation
> - religated vector (my CIPing never seems to be complete)
>
> What I got (according to restriction analysis) was:
> - religated vector (8 out of 24 minipreps)
> - vector containing TWO inserts in tandem orientation (16 out of 24
> minipreps)
>
> Any idea how this could happen?
>
> TIA, Martin
>
>
> ______________________________
> Martin Hoehne
> Institut fuer Biologie III
> Universitaet Freiburg, Germany






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