Nothern with 290bp probe

[JL Martinez]

    I have not problems with probes of 176 bp (I have used also
oligonucleotides and I obtain less signal but visible).  My RNAs are very
rich (they come from repeated DNA).  I perform hybridizations with 20% SDS,
disodium phosphate at 65 ºC and I obtain a good signal.  My probes are
dig-labeled.

Jose Luis

Departamento de Biologia Celular y del Desarrollo
Centro de Investigaciones Biologicas
Madrid-Spain



<Beat Reidy> escribió en el mensaje de noticias
38ad117f.12964331 at news.ethz.ch...
> Hi all
> I would like to perform a Northern blot with a relatively short probe
> of 290bp. GC content is about 60%. Any experience with with similar
> probes under standard hyb conditions (65°)?
>
> Thanks
> Beat
> beat.reidy at ipw.agrl.ethz.ch