DNA sequencing apparatus

Rafael Maldonado rmaldonado at ua.es
Fri Feb 18 16:38:08 EST 2000



"Dr P. Brennan" wrote:
>
>     Does anybody think this experience is unusual? Did we miss a step?
> Should we try a different apparatus that someone can recommend that
> never/rarely leaks?

Here is my method:

1. Siliconize both plates (or glue one of them, if you want/can to
expose to film with the plate)
2. Clamp both plates together with the spacers. Put the plates totally
horizontal, on something, as a tube rack, tips box, etc. Don't use any
tape.
3. Pour the acrilamide with a syringe, slowly. The superficial tension
will fill up the plateds for you.

No dripping, no mess.

-Rafa

-- 
Rafael Maldonado
Divison of Genetics
University of Alicante (Spain)




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