P. aeruginosa transformation

Arnoud van Vliet avvliet at knoware.nl
Sat Feb 19 10:23:34 EST 2000


You will have to give more info, on sieze of plasmid, which selection etc.

Anyway, best advice might be to try electroporation

Arnoud


From: Yong Jiang <yjiang at ucsd.edu>
Newsgroups: bionet.molbio.methds-reagnts
Sent: vrijdag 18 februari 2000 20:50
Subject: P. aeruginosa transformation


> Hi,
> I am transforming P. aeruginosa with plasmid which can replicate in it by
> CaCl2 mediate method. Everytime I spread the no DNA transformed cells on
> antibiotic plate, cells grow full of the plate. Could you give me amy
> suggestion?
> Best Regards!
> Yong
>
>







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