Using antibiotics when preparing competent cells

ajordaan at my-deja.com ajordaan at my-deja.com
Sun Feb 20 12:47:57 EST 2000


Hallo!  When preparing competent E.coli from
strains that have resistance to some antibiotic
(such as Xl1-blue) using Inoue's method, would it
influence the competency of the cells if the
antibiotic is included in the SOB medium in which
the cells are grown to mid-log phase?


Anton Jordaan
anton.jordaan at fabi.up.ac.za


Sent via Deja.com http://www.deja.com/
Before you buy.




More information about the Methods mailing list