E. coli XL1-Red cells

winter moon wintermoon_00 at yahoo.com
Sun Feb 20 13:05:11 EST 2000


Hi everyone,

I heard that Stratagene has the XL1-Red mutator strain
to generate random mutations.  The strain lacks three
of the primary DNA repair genes in E.coli, mutS, mutD,
and mutT, and makes its mutation rate approximately
5,000-fold higher.  Does anybody have an experience
with this strain?  I am wondering if this strain is as
effective as other mutagenesis (chemical treatment,
PCR, etc.).

Thanks.
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