E.coli ori compability

eforsen at my-deja.com eforsen at my-deja.com
Mon Feb 21 04:30:17 EST 2000


Hi

I'm designing two plasmids that I want to cotransform into E. coli. I'm
going to use different antibiotic resistance markers on them, but I want
to be sure that both plasmids are equally replicated. Instead of using
the same ori I want to use two different oris. Does anyone have any views
on wich oris are compatible and work well together? The copy number
should be approx. the same for both plasmids.

Thanks in advance

Erik Forsén
Department of Biotechnology
Royal Institute of Technology
Stockholm, Sweden


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