DNA-conc. in solutions

a_firzinger at my-deja.com a_firzinger at my-deja.com
Mon Feb 21 05:42:08 EST 2000


I usually measure DNA-conc. by determining OD at 260nm and
280nm. Usually I assume 50µg/ml ds DNA at OD260 of 1.
However, I know that there is a formula existing, which
includes the OD measured at 280nm. Maybe someone of you
remembers it and could send it to me. I just know a
formula for protein concentration which includes
OD260nm and 280nm:
Prot.conc.(mg/ml)= E280 x 1,56 - E260 x 0,757

Regards,

Andreas :-)


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